The Bacterial Protein Expression Core is located on the third floor of the EEJMRB at the University of Utah in close proximity to the labs of Hill, Sundquist, and Kay. It is managed by Heidi Schubert and staffed by two Center-funded technicians. The facility provides efficient construction of expression vectors, testing of protein expression and solubility, protein purification, and characterization. The Bacterial Protein Expression Core facilities are integrated with operations of the Eukaryotic Protein Expression Core for bioinformatics, cloning, and protein purification, and there is also a close association with protein structure determination efforts in X-ray and Protein NMR cores. This core therefore facilitates structural and biochemical studies on targets identified by the Center members pursuing questions of HIV/Host biology.